Coding

Part:BBa_K4343103

Designed by: Xiner Liu   Group: iGEM22_NNU-China   (2022-10-09)


EhElo9

EhElo9 encodes ∆-9 elongase from Emiliania huxleyi which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

Heterologous expression of EhElo9 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

We submitted the coding sequence of Δ9-elongase from I. galbana to the BLASTX and got to know the Δ9-elongase from E. huxleyi share the same homologous sequence. Then we acquired the complete sequence of this protein by E. huxleyi CCMP1516 filter protein models, after which the DNA segment was codon optimized and expressed in plant species.

Reference

Petrie, J. R., Mackenzie, A. M., Shrestha, P., Liu, Q., Frampton, D. F., Robert, S. S., & Singh, S. P. (2010). ISOLATION OF THREE NOVEL LONG‐CHAIN POLYUNSATURATED FATTY ACID Δ9‐ELONGASES AND THE TRANSGENIC ASSEMBLY OF THE ENTIRE PAVLOVA SALINA DOCOSAHEXAENOIC ACID PATHWAY IN NICOTIANA BENTHAMIANA 1. Journal of Phycology, 46(5), 917-925.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 675
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 818


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Categories
Parameters
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